Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Applications:

ELISA, WB, IHC

Name of antibody:

MAPK1/MAPK3

Immunogen:

Synthetic peptide of human MAPK1/MAPK3

Full name:

mitogen-activated protein kinase 1/3

Synonyms:

ERK1, ERK2, ERK1/2

SwissProt:

P28482/P27361

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human thyroid cancer and Human brain

IHC Recommend dilution:

50-200

WB Predicted band size:

41 kDa; 43 kDa

WB Positive control:

293T cells and Human brain malignant glioma tissue, Mouse brain and Human hepatocellsular carcinoma tissue

WB Recommended dilution:

500-2000