Background:

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Applications:

WB, IHC, IF

Name of antibody:

MAP2K1 (Phospho-Ser221)

Immunogen:

Synthetic peptide of human MAP2K1 (Phospho-Ser221)

Full name:

mitogen-activated protein kinase kinase 1 (Phospho-Ser221)

Synonyms：

MEK1; MKK1; MAPKK1; PRKMK1

SwissProt:

Q02750

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

45 kDa

WB Positive control:

Hela cells untreated or treated with EGF

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200