Calmodulin Rabbit Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1435
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Calmodulin
Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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17.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Calmodulin
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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801/805/808(Human);
12313/12314/12315(Mouse); 24242/24244/ 50663(Rat)
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SwissProt
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P62158(Human);
P62204(Mouse); P62161(Rat)
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Synonyms
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CALML2; CAMI;
CPVT4; DD132; PHKD; caM
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Category
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Neuronal
Signaling
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Background
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The level of
intracellular calcium is tightly regulated in all eukaryotic cells. A modest
increase in this level can result in a myriad of physiological responses,
most of which are mediated by calmodulin (CaM), the universal calcium sensor.
CaM directly modulates the activity of protein kinases and phosphatases, ion
channels and nitric oxide synthetases. It is generally involved in such
diverse processes as cell proliferation, endocytosis, cellular adhesion, protein
turn over and smooth muscle contraction. CaM (calmodulin) is an acidic
protein, 148 amino acids in length, with four helix-loop-helix calcium
binding domains. In humans, 3 distinct genes have been identified (CALM1,
CALM2 and CALM3); each encoding the identical protein. CALML3
(calmodulin-like 3, or calmodulin-related protein NB-1) shares significant
sequence identity with CaM and it is suggested that it may competitively bind
CaM substrates. Interestingly, CaM has been shown to associate with the carboxy
terminus of the dystrophin gene product, implying that it may regulate its
activity.
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY1435
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Calmodulin
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Calmodulin
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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