MCL1 Rabbit Monoclonal Antibody
- 產(chǎn)品名稱：MCL1 Rabbit Monoclonal Antibody
- 產(chǎn)品型號：
- 產(chǎn)品廠商：XYbscience
- 產(chǎn)品文檔：
MCL1 Rabbit Monoclonal Antibody
的詳細介紹
MCL1 Rabbit Monoclonal Antibody
|
產(chǎn)品編號 |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價格 |
|
XY1390 |
MCL1 Rabbit Monoclonal Antibody |
50μl |
618.00元 |
|
來源 |
用途 |
交叉反應(yīng)性 |
分子量 |
|
Rabbit |
WB, IP, IF, IHC, ICC, FC |
H, M, R |
37/29 kDa |
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
|
WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
|
1:1,000 |
1:10-1:50 |
1:50-1:200 |
1:50-1:200 |
1:50-1:200 |
1:50-1:100 |
- |
- |
抗體詳細信息如下:
|
About this Antibody |
|
|
Name |
MCL1 Rabbit Monoclonal Antibody |
|
Category |
Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
|
Isotype |
IgG |
|
Purification |
XYfinity purification |
|
About the Immunogen |
|
|
Immunogen |
Recombinant protein |
|
Gene ID |
4170(Human); 17210(Mouse); 60430(Rat) |
|
SwissProt |
Q07820(Human); P97287(Mouse); Q9Z1P3(Rat) |
|
Synonyms |
BCL2L3; EAT; MCL1-ES; MCL1L; MCL1S; Mcl-1; TM; bcl2-L-3; mcl1/EAT |
|
Category |
Apoptosis |
|
Background |
B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice. |
包裝清單:
|
產(chǎn)品編號 |
產(chǎn)品名稱 |
包裝 |
|
XY1390 |
MCL1 Rabbit Monoclonal Antibody |
50μl |
|
XY050 |
Western一抗稀釋液 |
50ml |
|
- |
說明書 |
1份 |
保存條件:
MCL1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(xué)(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。