Recombinant Human EMAP-II
產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY381-1mg
1mg
20294.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
9255
Q12904
E. coli
166aa
18.2kDa
－
About this Antibody
Name
Recombinant Human EMAP-II (Recombinant Human Endothelial-Monocyte Activating Polypeptide II; rHuEMAP-II)；重組人內(nèi)皮單核細(xì)胞活化多肽II
Synonyms
AIMP1; SCYE1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; EMAP-2; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte activating polypeptide II; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40ng/ml, corresponding to a specific activity of >2.5×104IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuEMAP-II as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Endothelial-Monocyte Activating Polypeptide II (EMAP-II) is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.
Amino Acid Sequence
SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK
包裝清單:
包裝
XY381-5μg
5μg
XY381-20μg
20μg
XY381-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。