beta-Actin抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號(hào)bs-0061R
英文名稱beta-Actin (Loading Control)
中文名稱β-肌動(dòng)蛋白/β-Actin 抗體（內(nèi)參抗體）
別 名Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.
JournalPMIDIFApplicationBiomaterials (2013)238100817.6040WBBiomaterials (2013)237779167.6040WBNanotoxicology (2015)263995856.4110WBArteriosclerosis, thrombosis, and vascular biology (2008)185997966.3380WBArchives of toxicology (2012)224223405.2150WBMolecular Neurobiology (2015)263855765.1370WBMolecular Cancer (2013)242156325.1300WBBritish Journal of Pharmacology (2014)248353595.0670 WB Endocrinology (2015) 26287402 4.5030 WB Molecular Pharmacology (2013)234702874.4110WBCancer letters (2007)182268554.2580WBBiochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2010)203822594.1340WBNanotechnology (2013)234259413.8420WB Nanotechnology (2013) 23425941 3.8420 WB Proteomics (2016) 27173832 3.8100 WB The Journal of Nutritional Biochemistry (2014) 24794015 3.7940 WB PloS One (2012) 23028660 3.7300 WB PloS One (2013) 23922700 3.7300 WB PloS One (2013) 24098763 3.7300 WB Cancer Immunology, Immunotherapy (2013) 24077852 3.6370 WB Colloids and Surfaces B: Biointerfaces (2012) 22503631 3.5540 WB PLoS One (2015) 26266800 3.5340 WB Cellular Physiology and Biochemistry (2014) 24556756 3.4150 WB Free radical research (2007) 17907002 3.2790 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB/IP Developmental & Comparative Immunology (2013) 23545285 3.2380 WB Developmental & Comparative Immunology (2013) 23500512 3.2380 WB Developmental & Comparative Immunology (2013) 23932982 3.2380 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB International Journal of Biological Sciences (2014) 24719562 3.1680 WB Toxicology Letters (2011) 21232584 3.1450 WB Neuroscience (2011) 21219973 3.1220 WB Diabetes/Metabolism Research and Reviews (2012) 21928330 2.9680 WB Chemico-Biological Interactions (2012) 24025711 2.9670 WB Fish & Shellfish Immunology (2012) 23063540 2.9640 WB Journal of Cancer Research and Clinical Oncology (2012) 22806311 2.9140 WB Food and Chemical Toxicology (2016) 27237588 2.9000 WB Brain Research Bulletin (2016) 26876758 2.7180 WB Life Sciences (2016) 26924495 2.7020 WB Nutrition and Cancer (2014) 24660968 2.6950 WB Neurochemistry international (2013) 24220540 2.6590 WB Neurochemistry international (2013) 24274996 2.6590 WB Neurochemistry international (2011) 21185898 2.6590 WB Life Science Journal (2013) Others 2.5550 WB Toxicology and Applied Pharmacology (2016) 27105818 2.5320 WB Tumor Biology (2013) 23539435 2.5180 WB Acta Pharmacologica Sinica (2015) 25937633 2.4960 WB World Journal of Gastroenterology (2015) 25663771 2.4330 WB Biochemical and Biophysical Research Communications (2013) 24211202 2.4060 WB Acta Pharmacologica Sinica (2014) 24632846 2.3540 WB Acta Pharmacologica Sinica (2014) 24909515 2.3540 WB Molecular and Cellular Biochemistry (2011) 21553022 2.3290 WB Molecular and Cellular Biochemistry (2014) 24833465 2.3290 WB Rheumatology international (2010) 19847436 2.2140 WB Medical Oncology (2013) 23536000 2.1470 WB Reproductive Biology and Endocrinology (2013) 23452984 2.1400 WB Neurochemical Research (2013) 23780673 2.1250 WB Virology Journal (2011) 21392403 2.0900 WB Respiratory physiology & neurobiology (2013) 23665052 2.0510 WB International Journal of Clinical and Experimental Pathology (2015) 26261494 1.8910 WB Research in Veterinary Science (2012) 23164636 1.7740 WB Cellular Immunology (2013) 23774531 1.7430 WB Applied Biochemistry and Biotechnology (2016) 27193254 1.7400 WB Poultry Science (2014) 24570433 1.7280 WB Nephrology (2011) 21771176 1.6880 WB Biological Trace Element Research (2015) 25876085 1.6080 WB Biological trace element research (2013) 23575899 1.3070 WB Others 1.2770 WB Brazilian Journal of Medical and Biological Research (2014) 25517918 1.1390 WB Experimental & Clinical Cardiology (2013) 23940455 1.1000 WB World Journal of Surgical Oncology (2014) 24885858 1.0900 WB CHINESE MEDICAL JOURNAL-BEIJING-ENGLISH EDITION (2009) 19236815 0.9010 WB Pediatrics International (2016) 27097979 0.7300 WB Experimental and Therapeutic Medicine (2014) 24396436 0.3440 WB
規(guī)格價(jià)格50ul/580元 購買 100ul/920元 購買 200ul/1600元 購買 大包裝/詢價(jià)
說 明 書50ul 100ul 200ul
產(chǎn)品類型內(nèi)參抗體
研究領(lǐng)域腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞骨架
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
beta-Actin抗體產(chǎn)品應(yīng)用WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
beta-Actin抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
beta-Actin抗體溶解方法：
1、在我們公司購買抗體后，查看標(biāo)簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內(nèi)，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標(biāo)簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內(nèi)，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內(nèi)，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標(biāo)簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標(biāo)簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlbeta-Actin抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復(fù)凍溶。
另：預(yù)試驗(yàn)可做幾個(gè)梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個(gè)*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí)，再使用對(duì)應(yīng)的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個(gè)月有效；若在-20℃下保存（推薦），至少三年有效。
beta-Actin抗體關(guān)聯(lián)產(chǎn)品列表：
bs-0952R No 合格 LH 促黃體生成素抗體
bs-0954R YES 合格 ET-1 內(nèi)皮素-1抗體
bs-0961R No 合格 合格 Leptin receptor 瘦素受體抗體
bs-0962R No 合格 SLC34A2 磷酸鈉協(xié)同轉(zhuǎn)運(yùn)蛋白抗體
bs-0975R YES 合格 SP1 轉(zhuǎn)錄生長因子SP1抗體
bs-0979R No 合格 CD38 CD38抗體
bs-0980R No 合格 CD38 CD38抗體
bs-0982R YES 合格 合格 phospho-NFKB p65(Ser536) 磷酸化細(xì)胞核因子抗體
bs-0988R No 合格 合格 CD71/Transferrin receptor 轉(zhuǎn)鐵蛋白受體抗體
bs-0990R YES 合格 HPV16 E6 protein 人類**狀瘤病毒16抗體
bs-0991R No 合格 HPV18 E6 protein 人類**狀瘤病毒18 E6抗體
bs-10005R YES 合格 c-Kit 干細(xì)胞生長因子受體/細(xì)胞表面分化抗原抗體
bs-10006R YES 合格 合格 CTLA4 細(xì)胞毒性T細(xì)胞抗原-4抗體
bs-10007R No 合格 EGFR 表皮生長因子受體抗體
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