BNP抗體經(jīng)ICC, IHC, WB, FACS嚴格驗證,品質保證.廣泛種屬反應性附有用戶反饋信息.確保特異性!**的技術支持幫您更快得到實驗結果，現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴格質控,特異性高,可重復性強,驗證數(shù)..可與小鼠, 大鼠, 人樣本反應并經(jīng)WB, IHC, ICC實驗嚴格驗證。所有產(chǎn)品均提供質保服務,中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-4865R
英文名稱BNP
中文名稱腦鈉素/利鈉肽抗體
別 名BNP 32; BNP(1-28); BNP(1-29); BNP(1-30); BNP(1-32); BNP(2-31); BNP(3-29); BNP(3-30); BNP(3-32); BNP(4-27); BNP(4-29); BNP(4-30); BNP(4-31); BNP(4-32); BNP(5-29); BNP(5-31); BNP(5-32); Brain natriuretic peptide 32; Brain type natriuretic peptide; Gamma brain natriuretic peptide; Natriuretic peptide B; Natriuretic peptide brain type; Natriuretic peptide precursor B; natriuretic peptide, brain type; ANFB_HUMAN; Natriuretic peptides B; natriuretic protein; NPPB; NPPB protein.
規(guī)格價格100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價
說 明 書100ul 200ul
研究領域心血管 信號轉導 生長因子和**
抗體來源Rabbit
克隆類型Polyclonal
BNP抗體交叉反應 Human, Mouse, Rat,
產(chǎn)品應用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
BNP抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
BNP抗體溶解方法：
1、在我們公司購買抗體后，查看標簽上的規(guī)格，如：0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例（部分抗體為液體，無需再溶解）：
溶解方法一：用移液器加0.1ml雙蒸水到試管內，混勻，-20℃保存；
若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加與標簽上規(guī)格一致的（如：0.2ml）雙蒸水溶解，混勻，-20℃保存。
溶解方法二：用移液器加 0.05ml（規(guī)格的 50%）雙蒸水到試管內，再加 0.05ml（規(guī)格的 50%）
的甘油到試管內，混勻，-20℃保存； 若為其它規(guī)格（如：0.2ml）的凍干粉抗體，則加標簽上規(guī)格的 50%（如：0.1ml）雙蒸水，再加標簽上規(guī)格的50%（如：0.1ml）甘油溶解，混勻，-20℃保存。
3、抗體的分裝
抗體溶解后，將其分裝成 5-10支（以0.1mlBNP抗體為例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反復凍溶。
另：預試驗可做幾個梯度1：100、1：200、1：400稀釋，如果背景高，還可再稀釋，選一個*佳的稀釋比例，再正式做，效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。
我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（推薦），至少三年有效。
BNP抗體關聯(lián)產(chǎn)品列表：
bs-0680R No 合格 IGF1R 胰島素樣生長因子1受體抗體
bs-0681R YES 合格 Insulin Receptor 胰島素受體抗體
bs-0683R YES 合格 合格 LRRK2 富亮氨酸重復激酶2抗體
bs-0684R YES 合格 合格 CD147 細胞外基質金屬蛋白酶誘導因子抗體
bs-0685R No 合格 合格 SYK 非受體型酪氨酸蛋白激酶抗體
bs-0686R No 合格 PTEN 一種腫瘤抑制基因抗體（C端）
bs-0687R No 合格 OAS1 寡腺苷酸合成酶-1
bs-0689R YES 合格 PEPT1 腸道肽轉運蛋白1/小肽轉運蛋白1抗體
bs-0701R YES 合格 Ceramide glucosyltransferase 谷氨酸半胱氨酸γ合成酶抗體
bs-0705R YES 合格 合格 LDL receptor 低密度脂蛋白受體抗體
bs-0708R YES 合格 NF-H 高分子量神經(jīng)絲蛋白抗體
bs-0709R YES 合格 Collagen II Ⅱ型膠原α1蛋白/軟骨鈣素抗體
bs-0710R YES 合格 合格 NF-M 中分子量神經(jīng)絲蛋白抗體
bs-0711R No 合格 FAM3B 胰腺衍生因子抗體
bs-0715R No 合格 Beta Tubulin 微管蛋白抗體
bs-0718R YES 合格 Smad2 細胞信號轉導分子Smad-2抗體
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