GLP-1 抗體經(jīng)ICC, IHC, WB, FACS嚴(yán)格驗(yàn)證,品質(zhì)保證.廣泛種屬反應(yīng)性附有用戶反饋信息.確保特異性!**的技術(shù)支持幫您更快得到實(shí)驗(yàn)結(jié)果,現(xiàn)貨.我司提供8萬種科研用專業(yè)抗體,經(jīng)嚴(yán)格質(zhì)控,特異性高,可重復(fù)性強(qiáng),驗(yàn)證數(shù)..可與小鼠, 大鼠, 人樣本反應(yīng)并經(jīng)WB, IHC, ICC實(shí)驗(yàn)嚴(yán)格驗(yàn)證。所有產(chǎn)品均提供質(zhì)保服務(wù),中國75%以上現(xiàn)貨。
產(chǎn)品編號bs-0038R
英文名稱GLP-1 (7-36)
中文名稱胰高血糖素樣肽-1抗體
別 名GCG; GLP 1; glucagon; Glucagon like peptide 1; GRPP; GLP-1(7-36); GLP-1(7-37); Oxyntomodulin; OXM; OXY; GLUC_HUMAN.
規(guī)格價(jià)格50ul/780元 購買 100ul/1280元 購買 200ul/1980元 購買 大包裝/詢價(jià)
說 明 書50ul 100ul 200ul
研究領(lǐng)域腫瘤 心血管 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 糖尿病 ***病 新陳代謝
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Sheep,
GLP-1 抗體產(chǎn)品應(yīng)用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
GLP-1 抗體產(chǎn)品介紹background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
GLP-1 抗體溶解方法:
1、在我們公司購買抗體后,查看標(biāo)簽上的規(guī)格,如:0.1ml、0.2ml
2、 以規(guī)格為0.1ml的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加0.1ml雙蒸水到試管內(nèi),混勻,-20℃保存;
若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加與標(biāo)簽上規(guī)格一致的(如:0.2ml)雙蒸水溶解,混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規(guī)格的 50%)雙蒸水到試管內(nèi),再加 0.05ml(規(guī)格的 50%)
的甘油到試管內(nèi),混勻,-20℃保存; 若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加標(biāo)簽上規(guī)格的 50%(如:0.1ml)雙蒸水,再加標(biāo)簽上規(guī)格的50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
3、抗體的分裝
抗體溶解后,將其分裝成 5-10支(以0.1mlGLP-1 抗體為例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反復(fù)凍溶。
另:預(yù)試驗(yàn)可做幾個(gè)梯度1:100、1:200、1:400稀釋,如果背景高,還可再稀釋,選一個(gè)*佳的稀釋比例,再正式做,效果*好。工作液可用“抗體稀釋液”稀釋。
后續(xù)試驗(yàn)時(shí),再使用對應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(推薦),至少三年有效。
GLP-1 抗體關(guān)聯(lián)產(chǎn)品列表:
bs-0623R YES 合格 合格 Cyclin D1 周期素D1抗體
bs-0624R No 合格 合格 CD272 B和T**細(xì)胞衰減蛋白抗體
bs-0629R No 合格 Estrogen Sulfotransferase 雌**硫酸轉(zhuǎn)移酶抗體
bs-0630R No 合格 Angiotensin II Type 1 Receptor 血管緊張素Ⅱ-1型受體/抗體
bs-0633R YES 合格 合格 CDK4 周期素依賴性激酶4抗體
bs-0634R YES 合格 合格 Aquaporin 4 水通道蛋白4抗體
bs-0635R No 合格 合格 TRIM21 核糖核蛋白自身抗原抗體
bs-0636R YES 合格 Phospho-P38 MAPK (Thr180 + Tyr182) 磷酸化-絲裂原活化蛋白激酶p38抗體(P-p38 MAPK)
bs-0637R YES 合格 P38 MAPK 絲裂原活化蛋白激酶p38α抗體
bs-0638R YES 合格 合格 TGF beta Receptor I 轉(zhuǎn)移生長因子β受體1抗體
bs-0641R YES 合格 Integrin alpha 4 整合素α4抗體
bs-0642R No 合格 MAdCAM-1 粘膜血管定居因子抗體
bs-0645R No 合格 Rat secretory IgA 分泌型**球蛋白A抗體
bs-0646R YES 合格 合格 CD34 CD34抗體
bs-0647R YES 合格 CD4 CD4抗體
bs-0648R YES 合格 CD8 CD8抗體
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