Hs 616.T細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 16 years
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-7378?
相關(guān)**： 其他**
Designations: Hs 616.T
Biosafety Level: 1
Hs 616.T細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Disease: Hodgkin's lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: modal number = 46; range = 45 to 46
Age: 16 years
Gender: male
Ethnicity: Hs 616.T細(xì)胞Caucasian
Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Hs 616.T細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium Hs 616.T細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
