C-4 II細胞

數(shù)量： 大量

ATCC Number： CRL-1595?

相關**： 腫瘤

器官來源： 宮頸

細胞形態(tài)： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

年限： 41 years

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

Designations: C-4 II

Depositors: N Auersperg

C-4 II細胞Biosafety Level: 2 [Cells contain human papilloma virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cervix

Disease: carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11,12

D16S539: 11

D5S818: 9,11

D7S820: 10,11

THO1: 9,9.3

C-4 II細胞TPOX: 10,11

vWA: 14

Isoenzymes: G6PD, B

Age: 41 years

Gender: female

Ethnicity: Caucasian

Comments: This is one of two lines developed from this patient (see ATCC CRL 1594).

The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.

Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 3 times per week

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

References: 22514: C-4 II細胞Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

26098: Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380

26099: Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382

26100: Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250

26101: Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980.C-4 II細胞 PubMed: 6768754