Jiyoye細(xì)胞
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： CCL-87?
相關(guān)**： **瘤
細(xì)胞類(lèi)型： B**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
年限： 7 years
Designations: Jiyoye [Jijoye, P-2003, P-3J]
Jiyoye細(xì)胞Depositors: W Henle
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: Epstein-Barr virus (EBV)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The cells carry and produce Epstein-Barr virus (EBV).
Virus Resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
Antigen Expression: HLA A32, B17, Bw37
DNA Profile (STR): Jiyoye細(xì)胞Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isoenzymes: G6PD, B
Age: 7 years
Gender: male
Ethnicity: Black, African
Comments: The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Propagation: Jiyoye細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 to 6 X 10 exp5 viable cells/ml.
A maximum of 2 - 3 X 10 exp6 viable cells/ml is obtainable.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1484
derivative:ATCC HTB-62
References: 22246: . . J. Clin. Pathol. 38: 261, 1985.
26255: Kohn G, et al. Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J. Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732
26256: . . In Vitro 4: 165, 1969.
32796: Jiyoye細(xì)胞Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082
