TUR細(xì)胞
ATCC Number： CRL-2367?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
相關(guān)**： 其他**
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
年限： 37 years
細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: TUR
Depositors: R Hass, I Prudovsky
TUR細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: monocyte
Source: Disease: histiocytic lymphoma
Cell Type: histocyte;
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Antigen Expression: HLA DR
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
TUR細(xì)胞vWA: 14,16
Age: 37 years
Gender: male
Ethnicity: Caucasian, White
Comments: TUR (TPA-U937-Resistant) is a stably transfected cell line generated by Ralf Hass and Masanori Hirano in 1992 from U-937 (ATCC CRL-1593.2), a monoblastoid cell line.
U-937 cells were transfected by electroporation with the pMG2neoPA plasmid containing the neomycin resistant gene and then selected in medium containing G418 and 12-O-tetradecanoylphorbol-13-acetate (TPA). [38600]
TUR cells have remained resistant to both G418 and TPA after culture in the absence of these agents for over 100 passages. [38600]
The cell line retains monocytic properties but does not differentiate along the macrophage pathway by phorbal ester treatment. [38600]
While treatment of human U-937 myeloid leukemia cells with TPA is associated with growth arrest and induction of monocytic differentiation, the TUR cell line is unresponsive to the growth-inhibitory effects of this agent. [38600]
The TUR variant is defective in TPA-induced signaling events upstream to activation of Raf-1 kinase. [38600]
Expression of major histocompatibility complex (MHC) Class II antigens on U937 and TPA-treated U937 cells is barely detectable.
However, there was a significantly constitutive expression of MHC class II, particularly human lymphocyte antigen (HLA-DR) on the surface of TUR and TPA-treated TUR cells.TUR細(xì)胞 [38602]
TUR cells continue to proliferate in the presence of TPA although increasing concentrations of TPA continuously reduces the proliferative capacity of the cells.
Unlike U-937 cells, exposure to TPA did not induce detectable levels of internucleosomal DNA fragmentation or the generation of apoptotic bodies. [38602]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 38600: TUR細(xì)胞Hass R, et al. Resistance to phorbol ester-induced differentiation of a U-937 myeloid leukemia cell variant with a signaling defect upstream to Raf-1 kinase. Cell Growth Differ. 4: 657-663, 1993. PubMed: 8398907
38602: Hass R, et al. Characterization of human TUR leukemia cells: continued cell cycle progression in the presence of phorbol ester is associated with resistance to apoptosis. Eur. J. Cell Biol. 65: 408-416, 1994. PubMed: 7720732
40046: Hass R, Lopez-Guerrero JA. Aggressive tumor growth of human TUR leukemia cells is associated with high levels of c-myc expression and down-regulation of p20-max. Int. J. Cancer 72: 1113-1116, 1997. PubMed: 9378547
40047: Meinhardt G, et al. Signaling defect in the activation of caspase-3 and PKCdelta in human TUR leukemia cells is associated with resistance to apoptosis. Exp. Cell Res. 247: 534-542, 1999. PubMed: 10066381
