EB-3細胞
年限： 3 years
細胞類型： B**細胞
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CCL-85?
相關(guān)**： **瘤
運輸方式： 凍存運輸
細胞形態(tài)： **樣
生長狀態(tài)： 懸浮生長
Designations: EB-3 [EB3]
EB-3細胞Depositors: W Henle
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
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Virus Resistance: partially resistant to polioviruses and vesicular stomatitis virus
Antigen Expression: HLA A3, Aw32, Cw2
DNA Profile (STR): EB-3細胞Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
Isoenzymes: G6PD, A
Age: 3 years
Gender: male
Ethnicity: Black
Comments: EBNA positive.
Propagation: EB-3細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
26251: . . Trans. N.Y. Acad. Sci. 29: 71, 1966.
26252: . . Wistar Inst. Symp. EB-3細胞Monogr. 4: 59, 1965.
