NCI-H747細胞

運輸方式： 凍存運輸

器官來源： 盲腸

是否是腫瘤細胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

年限： 69 years

ATCC Number： CCL-252?

數量： 大量

相關**： 大腸癌

細胞形態(tài)： 上皮樣

Designations: NCI-H747 [H747]

Depositors: AF Gazdar

NCI-H747細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, floating aggregates of round cells with some attached cells

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cecum

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA), 8 ng/ml per 10 exp6 cells per 10 days

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H747細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: Blood type A; Rh+; CA19-9 antigen

Cytogenetic Analysis: modal number = 66; range = 63 to 75

This is a hypotriploid human cell line. The modal chromosome number is 66, occurring in 20% of cells. The rate of higher ploidies is 1.6%. Twelve marker chromosomes are common to all cells; these include single copy for i(1q), i(7p), i(10q); two copies for i(3q) and two to three copies for der(12)t(12;13)(q24,33;q12). The N12, N13, X and Y have single copy each. The presence of the Y was confirmed by Q-banding.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 69 years

Gender: male

Ethnicity: Caucasian

Comments: NCI-H747細胞This line was derived from a metastasis in a common duct node from a patient prior to therapy.

The cells are negative for Dopa decarboxylase and TAG-72 antigen but are positive for CEA and CA19-9 antigen.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO

Storage temperature: NCI-H747細胞liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249