NCI-H660細(xì)胞
細(xì)胞形態(tài)： 上皮樣
ATCC Number： CRL-5813?
相關(guān)**： 其他**
細(xì)胞類型： 其他細(xì)胞類型
年限： stage E
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 前列腺
數(shù)量： 大量
生長狀態(tài)： 漂浮生長
Designations: NCI-H660
Depositors: AF Gazdar, JD Minna
NCI-H660細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: floating and attached clusters
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: prostate
Tumor Stage: stage E
Disease: small cell carcinoma
Derived from metastatic site: lymph node
Cell Type: epithelial neuroendocrine;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H660細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
Receptors: atrial natriuretic peptide (ANP), expressed
Reverse Transcript: N
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 7,9.3
TPOX: 8
vWA: 17
Age: 63 years *****
Gender: male
Ethnicity: Caucasian
HeLa Markers: N
Comments: NCI-H660細(xì)胞While NCI-H660 has the appearance and many of the properties of small cell carcinoma, there are also differences. NCI-H660 expresses elevated levels of L-dopa carboxylase and bombesin-like immunoreactivity. The cells express functional atrial natriuretic peptide (ANP) receptors, but ANP addition produces no detectable change in the cells' growth pattern.
Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No.30-2001. To make the complete growth medium, add the following components to the base medium
0.005 mg/ml Insulin
0.01 mg/ml Transferrin
30nM Sodium selenite (final conc.)
10 nM Hydrocortisone (final conc.)
10 nM beta-estradiol (final conc.)
extra 2mM L-glutamine (for final conc. of 4 mM)
5% fetal bovine serum (final conc.)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Add medium as the cell density increases. Shake flask to dislodge attached cells. Single cells are often dead and clusters are viable. Subculture when there are numerous, healthy-appearing clusters present in suspension.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium;90% ; DMSO, 10%.
Storage temperature: liquid nitrogen vapor phase
Related Products: NCI-H660細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
recommended serum:ATCC 30-2020
L-Glutamine Solution, 200 mM:ATCC 30-2214
References: 22725: Lai SL, et al. Molecular genetic characterization of neuroendocrine lung cancer cell lines. Anticancer Res. 15: 225-232, 1995. PubMed: 7762988
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
23227: Johnson BE, et al. Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies. J. Natl. Cancer Inst. 81: 1223-1228, 1989. PubMed: 2569043
