產(chǎn)品描述
GATA-Luc熒光素酶報(bào)告基因（報(bào)告基因質(zhì)粒）（GATA luciferase reporter plasmid）是自主研發(fā)的用于檢測(cè)GATA轉(zhuǎn)錄活性水平為目的的報(bào)告基因。GATA(globin transcription factor)轉(zhuǎn)錄因子家族包括6鋅指結(jié)合蛋白調(diào)控細(xì)胞的增殖和分化。GATA家族成員涉及造血、心臟和消化道的發(fā)育。
GATA報(bào)告基因是用于檢測(cè)細(xì)胞中球蛋白轉(zhuǎn)錄因子家族的活性、**研究以及基因過表達(dá)和RNAi的表型分析等。
pGMGATA-Lu是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)GATA結(jié)合位點(diǎn)，可以高靈敏度地檢測(cè)GATA的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小，使得GATA報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。GATA luciferase reporter plasmid （GATA-Luc熒光素酶報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
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GATA luciferase reporter plasmid （GATA-Luc熒光素酶報(bào)告基因質(zhì)粒）質(zhì)粒元件信息
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GATA response element (GATA)
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32-73
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Minimal TA promoter (pTA)
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102-124
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Luciferase reporter gene
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156-1818
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SV40 late poly(A) signal
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1853-2074
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SV40 early promoter
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2122-2540
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Synthetic neomycin phosphotransferase(Neor) coding region
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2565-3359
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Synthetic poly(A) signal
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3384-3432
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Synthetic Beta-lactamase(Ampr) coding region
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4547-5407
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Synthetic poly(A) signal/transcriptional pause site
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5512-5665
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GATA response element序列信息
1 GGCCTAACTGGCCGGTACCGCTAGCCTCGATGGCATTCTCTATCTGATTG
51 TTGGCATTCTCTATCTGATTGTTGCGCGTAGATCTGCAGAAGCTTAGACA
pGMGATA-Lu質(zhì)粒測(cè)序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20oC保存。
注意事項(xiàng)
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。GATA luciferase reporter plasmid （GATA-Luc熒光素酶報(bào)告基因質(zhì)粒）
使用說明
pGMGATA-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
參考文獻(xiàn)
[1] Fujiwara T, et al. Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells. PLoS One. 2012;7(9):e40959.
[2] Chan YC, et al. Downregulation of endothelial microRNA-200b supports cutaneous wound angiogenesis by desilencing GATA binding protein 2 and vascular endothelial growth factor receptor 2. ArteriosclerThrombVasc Biol. 2012 Jun;32(6):1372-82.
[3] Warkman AS, et al. Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart. Cytoskeleton (Hoboken). 2012 May;69(5):324-35.GATA luciferase reporter plasmid （GATA-Luc熒光素酶報(bào)告基因質(zhì)粒）
[4] Ishijima Y, et al. GATA transcription factors are involved in IgE-dependent mast cell degranulation by enhancing the expression of phospholipase C-γ1. Genes Cells. 2012 Apr;17(4):285-301.
