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Species
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Rat
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Accession
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Q63086
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GeneID
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29201
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠心肌營(yíng)養(yǎng)素-1蛋白Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠心肌營(yíng)養(yǎng)素-1蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.
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Endotoxin
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Less than 1 EU/μg of rRtCT-1 as determined by LAL method.
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Reconstitution
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重組大鼠心肌營(yíng)養(yǎng)素-1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠心肌營(yíng)養(yǎng)素-1蛋白
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SDS-PAGE
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Reference
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1. Pennica D, Wood WI, Chien KR. 1996. Cytokine Growth Factor Rev. 7:81-91.
2. Robledo O, Fourcin M, Chevalier S, et al. 1997. J Biol Chem. 272:4855-63.
3. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.
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Background
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Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the ***** heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.
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