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Synonyms
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Small-inducible Cytokine A11
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Species
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Rat
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Accession
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P97545
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GeneID
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29397
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠嗜酸性粒細(xì)胞趨化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
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Quantity
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5μg/20μg/1000μg
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AA Sequence
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HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠嗜酸性粒細(xì)胞趨化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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重組大鼠嗜酸性粒細(xì)胞趨化因子/ CCL11蛋白Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠嗜酸性粒細(xì)胞趨化因子/ CCL11蛋白
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SDS-PAGE
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Reference
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1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12.
2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7.
3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56.
4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.
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Background
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CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.
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