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Synonyms
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Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC
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Species
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Rat
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Accession
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Q9QXY8
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GeneID
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287561
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Source
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重組大鼠單核細(xì)胞趨化蛋白3 / CCL7蛋白Escherichia coli.
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Molecular Weight
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Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP
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Purity
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重組大鼠單核細(xì)胞趨化蛋白3 / CCL7蛋白> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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重組大鼠單核細(xì)胞趨化蛋白3 / CCL7蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rRtMCP-3/CCL7 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠單核細(xì)胞趨化蛋白3 / CCL7蛋白
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SDS-PAGE
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Reference
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1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8.
2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.
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Background
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Rat CCL7, belonging to the CC chemokine family, is also named as MCP-3, because the sequence comparison suggested that the rat CCL7 is a homologue of the human MCP-3 and is postulated the rat MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic functions for monocytes and eosinophils, but not for neutrophils. Additionally, it can activated NK cells as well as CD4+ and CD8+ T lymphocytes. CCL7 was found to interact with MMP2.
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