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- 產(chǎn)品名稱：重組大鼠血小板衍生生長因子-AA蛋白
- 產(chǎn)品型號：rRtPDGF-AA
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時間：2018-07-12
- 在線詢價
簡單介紹
重組大鼠血小板衍生生長因子-AA蛋白與其它公司提供的重組蛋白不同，rRtPDGF-AA蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠血小板衍生生長因子-AA蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組大鼠血小板衍生生長因子-AA蛋白
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Species
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Rat
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Accession
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P28576
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GeneID
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25266
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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重組大鼠血小板衍生生長因子-AA蛋白MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
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Physical Appearance
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重組大鼠血小板衍生生長因子-AA蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
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Endotoxin
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Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重組大鼠血小板衍生生長因子-AA蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠血小板衍生生長因子-AA蛋白
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SDS-PAGE
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