- 如果您對該產(chǎn)品感興趣的話，可以
- 產(chǎn)品名稱：重組大鼠肝素結(jié)合性EGF樣生長因子蛋白
- 產(chǎn)品型號：rRtHB-EGF
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時間：2018-08-31
- 在線詢價
簡單介紹
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白與其它公司提供的重組蛋白不同，rRtHB-EGF蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠肝素結(jié)合性EGF樣生長因子蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白
|
Synonyms
|
HBEGF, DT-R
|
|
Species
|
Rat
|
|
Accession
|
Q06175
|
|
GeneID
|
25433
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
|
|
Quantity
|
10μg/50μg/1000μg
|
|
AA Sequence
|
DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
|
|
Endotoxin
|
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重組大鼠肝素結(jié)合性EGF樣生長因子蛋白
|
|
|
Reference
|
1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.
2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.
|
|
Background
|
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.
|
