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- 產(chǎn)品名稱：重組大鼠膠質細胞成熟因子beta蛋白
- 產(chǎn)品型號：rRtGMF-β
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關文檔
- 發(fā)布時間：2018-07-09
- 在線詢價
簡單介紹
重組大鼠膠質細胞成熟因子beta蛋白與其它公司提供的重組蛋白不同，rRtGMF-β蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠膠質細胞成熟因子beta蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組大鼠膠質細胞成熟因子beta蛋白
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Synonyms
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GMFB
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Species
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Rat
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Accession
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Q63228
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GeneID
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81661
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠膠質細胞成熟因子beta蛋白Approximately 16.6 kDa, a single non-glycosylated polypeptide chain containing 141 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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SESLVVCDVA EDLVEKLRKF RFRKETHNAA IIMKIDKDKR LVVLDEELEG VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPLGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Data is not available.
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Physical Appearance
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重組大鼠膠質細胞成熟因子beta蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rRtGMF-β as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重組大鼠膠質細胞成熟因子beta蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠膠質細胞成熟因子beta蛋白
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SDS-PAGE
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Reference
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1. Gandhi M, Smith BA, Bovellan M, et al. 2010. Curr Biol, 20: 861-7.
2. Roman AandTombarkiewicz B. 2009. Bioelectromagnetics, 30: 21-8.
3. Ohnishi T, Arita N, Hayakawa T, et al. 1993. Biochem Biophys Res Commun, 193: 518-25.
4. Nakano K, Kuwayama H, Kawasaki M, et al. 2010. Cytoskeleton (Hoboken), 67: 373-82.
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Background
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The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.
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