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Synonyms
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T-cell Activation Protein P600
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Species
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Rat
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Accession
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P42203
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GeneID
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116553
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠白細(xì)胞介素-13,113a.a.蛋白Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 113 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSMVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠白細(xì)胞介素-13,113a.a.蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.
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Endotoxin
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Less than 1 EU/μg of rRtIL-13, 113a.a. as determined by LAL method.
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Reconstitution
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重組大鼠白細(xì)胞介素-13,113a.a.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠白細(xì)胞介素-13,113a.a.蛋白
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SDS-PAGE
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Reference
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1. Schmutz J, Martin J, Terry A, et al. 2004. Nature, 431: 268-74.
2. Wynn TA. 2003. Annu Rev Immunol, 21: 425-56.
3. Moy FJ, Diblasio E, Wilhelm J, et al. 2001. J Mol Biol, 310: 219-30.
4. Lakkis FG, Cruet EN, Nassar GM, et al. 1997. Biochem Biophys Res Commun, 235: 529-32.
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Background
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Interleukin-13 (IL-13) is expressed by the IL13 gene and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Mature rat IL-13 shares 59 %, 75 %, and 60 % amino acid sequence identity with human, mouse, and rhesus IL-13, respectively
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