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Synonyms
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IL-1RN, IRAP, IL1 Inhibitor
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Species
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Rat
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Accession
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P25086
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GeneID
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60582
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠白細(xì)胞介素-1受體拮抗劑蛋白蛋白Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
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Quantity
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20μg/100μg/1000μg
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AA Sequence
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HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠白細(xì)胞介素-1受體拮抗劑蛋白蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg in the presence of 50 pg/ml rRtIL-1α.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.
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Reconstitution
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重組大鼠白細(xì)胞介素-1受體拮抗劑蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠白細(xì)胞介素-1受體拮抗劑蛋白蛋白
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SDS-PAGE
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Reference
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1. Dinarello CA. 1994. FASEB J. 8:1314-25.
2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6.
3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7.
4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94.
5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.
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Background
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IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89% and 73% a.a. sequence homology with murine and human IL-1RA.
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