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- 產(chǎn)品名稱：重組大鼠白細(xì)胞介素-22蛋白
- 產(chǎn)品型號(hào)：rRtIL-22
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時(shí)間：2018-06-24
- 在線詢價(jià)
簡單介紹
重組大鼠白細(xì)胞介素-22蛋白與其它公司提供的重組蛋白不同，rRtIL-22蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對(duì)蛋白的損傷降低到*小程度.重組大鼠白細(xì)胞介素-22蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
產(chǎn)品描述
重組大鼠白細(xì)胞介素-22蛋白
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Synonyms
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Cytokine Zcyto 18, TIF
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Species
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Rat
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Accession
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Q198B4
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Unigene
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Rn.195879.
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠白細(xì)胞介素-22蛋白Approximately16.6 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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LPINSQCKLE AANFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEELFRGV KAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSIHLSPCH ISGDDQNIQK NVRQLKETVQ KLGESGEIKA IGELDLLFMS LRNACV
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠白細(xì)胞介素-22蛋白Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rRtIL-22 as determined by LAL method.
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Reconstitution
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重組大鼠白細(xì)胞介素-22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠白細(xì)胞介素-22蛋白
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SDS-PAGE
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Reference
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1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.
2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.
3. Honda K. 2012. Immunity, 37: 952-4.
4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.
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Background
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IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant rat Interleukin-22 contains 146 amino acids and it has approximately 79 % and 92 % amino acid sequence identity with human and murine IL-22.
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