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Synonyms
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T-cell Growth Factor, CGF
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Species
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Rat
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Accession
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P17108
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GeneID
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116562
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Source
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Escherichia coli.
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Molecular Weight
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重組大鼠白細(xì)胞介素-2蛋白Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.
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Quantity
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5μg/20μg/1000μg
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AA Sequence
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APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組大鼠白細(xì)胞介素-2蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0.
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Endotoxin
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Less than 1 EU/μg of rRtIL-2 as determined by LAL method.
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Reconstitution
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重組大鼠白細(xì)胞介素-2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組大鼠白細(xì)胞介素-2蛋白
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SDS-PAGE
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Reference
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1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.
2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.
3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.
4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.
5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.
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Background
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IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.
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