- 如果您對該產(chǎn)品感興趣的話，可以
- 產(chǎn)品名稱：重組鼠單核細胞趨化蛋白-3 / CCL7蛋白
- 產(chǎn)品型號：rMuMCP-3/CCL7
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時間：2018-08-19
- 在線詢價
簡單介紹
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白與其它公司提供的重組蛋白不同，rMuMCP-3/CCL7蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠單核細胞趨化蛋白-3 / CCL7蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白
|
Synonyms
|
Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC
|
|
Species
|
Murine
|
|
Accession
|
Q03366
|
|
GeneID
|
20306
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
|
|
Quantity
|
2μg/10μg/1000μg
|
|
AA Sequence
|
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 2× PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/μg of rMuMCP-3/CCL7 as determined by LAL method.
|
|
Reconstitution
|
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重組鼠單核細胞趨化蛋白-3 / CCL7蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8.
2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.
|
|
Background
|
Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic function for monocytes and eosinophils, but not for neutrophils.
|
