- 如果您對該產(chǎn)品感興趣的話，可以
- 產(chǎn)品名稱：重組鼠促乳素蛋白
- 產(chǎn)品型號：rMuProlactin
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時間：2018-08-30
- 在線詢價
簡單介紹
重組鼠促乳素蛋白與其它公司提供的重組蛋白不同，rMuProlactin蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠促乳素蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組鼠促乳素蛋白
|
Species
|
Murine
|
|
Accession
|
P06879
|
|
Unigene
|
Mm.1270.
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重組鼠促乳素蛋白Approximately 22.4 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
|
|
Quantity
|
10μg/50μg/1000μg
|
|
AA Sequence
|
LPICSAGDCQ TSLRELFDRV VILSHYIHTL YTDMFIEFDK QYVQDREFMV KVINDCPTSS LATPEDKEQA LKVPPEVLLN LILSLVQSSS DPLFQLITGV GGIQEAPEYI LSRAKEIEEQ NKQLLEGVEK IISQAYPEAK GNGIYFVWSQ LPSLQGVDEE SKILSLRNTI RCLRRDSHKV DNFLKVLRCQ IAHQNNC
|
|
Purity
|
重組鼠促乳素蛋白> 98 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
重組鼠促乳素蛋白Less than 0.1 EU/μg of rMuProlactin as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重組鼠促乳素蛋白
|
|
|
SDS-PAGE
|
|
