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Synonyms
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STCP-1, MDC (1-69), Macrophage-derived Chemokine
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Species
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Murine
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Accession
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O88430
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GeneID
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20299
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Source
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Escherichia coli.
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Molecular Weight
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重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.
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Quantity
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5μg/20μg/1000μg
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AA Sequence
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GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human activated lymphocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rMuMDC/CCL22 as determined by LAL method.
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Reconstitution
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重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠巨噬細胞衍生的趨化因子/ CCL22蛋白
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SDS-PAGE
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Reference
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1. Nomiyama H, Imai T, Kusuda J, et al. 1998. Cytogenet Cell Genet, 81: 10-1.
2. Godiska R, Chantry D, Raport CJ, et al. 1997. J Exp Med, 185: 1595-604.
3. Yamashita UandKuroda E. 2002. Crit Rev Immunol, 22: 105-14.
4. Katou F, Ohtani H, Nakayama T, et al. 2001. Am J Pathol, 158: 1263-70.
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Background
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CCL22 is a protein that in mouse is encoded by the CCL22 gene, which locates on the Chr. 8. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).
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