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- 產(chǎn)品名稱：重組鼠單核細胞趨化蛋白-5 / CCL12蛋白
- 產(chǎn)品型號：rMuMCP-5/CCL12
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無相關(guān)文檔
- 發(fā)布時間：2018-07-20
- 在線詢價
簡單介紹
重組鼠單核細胞趨化蛋白-5 / CCL12蛋白與其它公司提供的重組蛋白不同，rMuMCP-5/CCL12蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠單核細胞趨化蛋白-5 / CCL12蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產(chǎn)品描述
重組鼠單核細胞趨化蛋白-5 / CCL12蛋白
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Synonyms
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MCP-1-related Chemokine, Small-inducible Cytokine A12
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Species
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Murine
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Accession
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Q62401
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GeneID
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20293
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Source
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重組鼠單核細胞趨化蛋白-5 / CCL12蛋白Escherichia coli.
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Molecular Weight
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Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
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Quantity
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5μg/20μg/1000μg
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AA Sequence
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GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組鼠單核細胞趨化蛋白-5 / CCL12蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
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Reconstitution
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重組鼠單核細胞趨化蛋白-5 / CCL12蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠單核細胞趨化蛋白-5 / CCL12蛋白
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Reference
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1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.
2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51.
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Background
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Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.
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