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Synonyms
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14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP
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Species
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Murine
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Accession
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P12710
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GeneID
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14080
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Source
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Escherichia coli.
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Molecular Weight
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重組鼠脂肪酸結(jié)合蛋白1蛋白Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
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Quantity
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5μg/25μg/1000μg
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AA Sequence
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MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組鼠脂肪酸結(jié)合蛋白1蛋白Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.
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Endotoxin
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Less than 1 EU/μg of rMuFABP1 as determined by LAL method.
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Reconstitution
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重組鼠脂肪酸結(jié)合蛋白1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠脂肪酸結(jié)合蛋白1蛋白
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SDS-PAGE
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Reference
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1. Chmurzynska A. 2006. J Appl Genet. 47:39-48.
2. Weisiger RA. 2002. Mol Cell Biochem. 239:35-43.
3. Chen SH, Van Tuinen P, Ledbetter DH, et al. 1986. Somat Cell Mol Genet. 12:303-6.
4. Weickert MO, Loeffelholz CV, Roden M, et al. 2007. Am J Physiol Endocrinol Metab. 293:E1078-84.
5. Bassuk JA, Tsichlis PN, Sorof S. 1987. Proc Natl Acad Sci U S A. 84:7547-51.
6. He Y, Yang X, Wang H, et al. 2007. Biochemistry. 46:12543-56.
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Background
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The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. Fatty acid-binding protein 1 (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP1 binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP1 shares 84 % and 94 % a.a. sequence identity with human and rat FABP1.
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