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Synonyms
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FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1
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Species
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Murine
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Accession
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Q9JLA2
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GeneID
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54448
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Source
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重組鼠白細(xì)胞介素-36α，160a.a.蛋白Escherichia coli.
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Molecular Weight
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Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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重組鼠白細(xì)胞介素-36α，160a.a.蛋白MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36α at 1 μg/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 μg/mL.
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Physical Appearance
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重組鼠白細(xì)胞介素-36α，160a.a.蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
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Endotoxin
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Less than 1 EU/μg of rMuIL-36α, 160a.a. as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠白細(xì)胞介素-36α，160a.a.蛋白
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SDS-PAGE
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Reference
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1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25.
2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973.
3. Barksby HE, Lea SR, Preshaw PM, et al. 2007. Clin Exp Immunol. 149:217-25.
4. Smith DE, Renshaw BR, Ketchem RR, et al. 2000. J Biol Chem. 275:1169-75.
5. Dunn E, Sims JE, Nicklin MJ, et al. 2001. Trends Immunol. 22:533-6.
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Background
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Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 160 amino acids residues which is a single non-glycosylated polypeptide. Specifically, mouse IL-36α shares 83 % a.a. sequence identity with rat IL-36α, 54-60 % with human, rabbit, equine and bovine IL-36α.
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