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Synonyms
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Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor.
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Species
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Murine
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Accession
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P01586
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GeneID
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16187
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Source
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重組鼠白細(xì)胞介素-3蛋白Escherichia coli.
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Molecular Weight
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Approximately 14.8 kDa globular protein containing 134 amino acid residues.
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Quantity
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2μg/10μg/1000μg
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AA Sequence
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DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC
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Purity
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重組鼠白細(xì)胞介素-3蛋白> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 × 107 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuIL-3 as determined by LAL method.
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Reconstitution
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重組鼠白細(xì)胞介素-3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠白細(xì)胞介素-3蛋白
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SDS-PAGE
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Reference
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1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.
2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.
3. Miyatake S, Yokota T, Lee F, et al. 1985. Proc Natl Acad Sci U S A. 82:316-20.
4. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.
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Background
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Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included α and β subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity.
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