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- 產(chǎn)品名稱(chēng)：重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白
- 產(chǎn)品型號(hào)：rMuFGF-17
- 產(chǎn)品展商：KALANG
- 產(chǎn)品文檔：無(wú)相關(guān)文檔
- 發(fā)布時(shí)間：2018-08-19
- 在線詢價(jià)
簡(jiǎn)單介紹
重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白與其它公司提供的重組蛋白不同，rMuFGF-17蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
產(chǎn)品描述
重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白
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Species
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Murine
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.
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AA Sequence
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TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg in the presence of 10 μg/ml of heparin.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.
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Endotoxin
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Less than 0.1 EU/μg of rMuFGF-17 as determined by LAL method.
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Reconstitution
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重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠成纖維細(xì)胞生長(zhǎng)因子17蛋白
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Background
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FGF-17 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. The mRNA of FGF-17 was found in midgestation of embryo and multiple ***** tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries.
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