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- 產品名稱：重組鼠白細胞介素-36γ，152a.a.蛋白
- 產品型號：rMuIL-36γ, 152a.a.
- 產品展商：KALANG
- 產品文檔：無相關文檔
- 發(fā)布時間：2018-07-07
- 在線詢價
簡單介紹
重組鼠白細胞介素-36γ，152a.a.蛋白與其它公司提供的重組蛋白不同，rMuIL-36γ, 152a.a.蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠白細胞介素-36γ，152a.a.蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
產品描述
重組鼠白細胞介素-36γ，152a.a.蛋白
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Species
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Murine
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Accession
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Q8R460
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Unigene
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Mm.249379.
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Source
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Escherichia coli.
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Molecular Weight
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重組鼠白細胞介素-36γ，152a.a.蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
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AA Sequence
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GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組鼠白細胞介素-36γ，152a.a.蛋白Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered solution in 1 M MOPS, 10 mM NaAC, pH7.6, with 2 mM EDTA, 5 % Trehalose, 0.02 % Tween-20.
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Endotoxin
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Less than 0.1 EU/μg of rMuIL-36γ, 152a.a. as determined by LAL method.
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Reconstitution
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重組鼠白細胞介素-36γ，152a.a.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組鼠白細胞介素-36γ，152a.a.蛋白
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Background
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Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36γ is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36γ also can function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Recombinant murine IL-36γ is synthesized as a 17.3 kDa, 152 amino acid (a.a.) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site. Murine to human, IL-36γ shares 53 % a.a. identity. Within the family, IL-36γ shares about 25 % ~ 55 % a.a. sequence identity with IL-1RA, IL-1β, IL-36RA, IL-36α, IL-37, IL-36β and IL-1F10.
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