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Synonyms
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TNFSF5, sCD40L, CD154, gp39, T-BAM, TNF-related Activation Protein, TRAP
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Species
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Human
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Accession
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P29965
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GeneID
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959
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Source
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Escherichia coli.
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Molecular Weight
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重組人可溶性CD40配體/ TNFSF5蛋白Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
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Quantity
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10μg/50μg/1000μg
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AA Sequence
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MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組人可溶性CD40配體/ TNFSF5蛋白Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
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Endotoxin
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Less than 1 EU/μg of rHusCD40L as determined by LAL method.
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Reconstitution
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重組人可溶性CD40配體/ TNFSF5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組人可溶性CD40配體/ TNFSF5蛋白
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Reference
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1. Li R, Chen WC, Pang XQ, et al. 2011. Mol Biol Rep, 38: 5459-64.
2. Reinboldt S, Wenzel F, Rauch BH, et al. 2009. Platelets, 20: 441-4.
3. Tousoulis D, Antoniades C, Nikolopoulou A, et al. 2007. Eur J Clin Invest, 37: 623-8.
4. Varo N, Libby P, Nuzzo R, et al. 2005. Diab Vasc Dis Res, 2: 81-7.
5. Holzer G, Pfandlsteiner T, Blahovec H, et al. 2003. Wien Med Wochenschr, 153: 40-2.
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Background
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CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
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