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Synonyms
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TNFSF13B, TNFSF20, TALL-1, BLys, THANK, B cell Activating Factor belonging to the TNF family
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Species
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Human
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Accession
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Q9Y275
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GeneID
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10673
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Source
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Escherichia coli.
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Molecular Weight
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重組人B細(xì)胞激活因子/ TNFSF13B蛋白Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
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Quantity
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5μg/20μg/1000μg
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AA Sequence
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MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重組人B細(xì)胞激活因子/ TNFSF13B蛋白Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 μg/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
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Endotoxin
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Less than 1 EU/μg of rHuBAFF as determined by LAL method.
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Reconstitution
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重組人B細(xì)胞激活因子/ TNFSF13B蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重組人B細(xì)胞激活因子/ TNFSF13B蛋白
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SDS-PAGE
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Reference
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1. Craxton A, Draves KE, Gruppi A, et al. 2005. J Exp Med, 202: 1363-74.
2. Kalled SL. 2005. Immunol Rev, 204: 43-54.
3. Kuo SH, Yeh PY, Chen LT, et al. 2008. Blood, 112: 2927-34.
4. Rowland SL, Leahy KF, Halverson R, et al. 2010. J Immunol, 185: 4570-81.
5. Liu ZandDavidson A. 2011. Exp Cell Res, 317: 1270-7.
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Background
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B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56 % a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.
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